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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 1207-1217
DOI: https://doi.org/10.20546/ijcmas.2017.604.148


Bacterial Community Analysis of Soybean (Glycine max) Sprayed with Panchagavya Revealed by DGGE
Biradar Balasaheb Gunwantrao1*, C.R. Patil2 and Malik Ahmed Pasha1
1Department of Biotechnology, University of Agricultural Sciences, Dharwad, India
2Department of Agricultural Microbiology, Institute of Organic Farming, University of Agricultural Sciences Dharwad, India
*Corresponding author
Abstract:

Present study was aimed to understand the bacterial community composition, diversity, richness, carrying capacity and population dynamics present on soybean leaf in response to Panchagavya sprays using DGGE (Denaturing Gradient Gel Electrophoresis) and molecular fingerprinting technique. The seeds of soybean variety JS-335 were collected, surface sterilized and used in a pot culture study. In-vivo pot experiment was carried out in four sets. First three sets were sprayed on 15th day after sowing (DAS), 30th DAS and at both 15th and 30th DAS respectively and the fourth set was kept as control without spraying throughout the experiment period. The metagenomic DNA was obtained in the range of 800 to 1214 ng /μl. The Shannon diversity, Range Weighted Richness and Pielou’s evenness values clearly indicated that spraying of Panchagavya twice at 15th and 30th DAS increased bacterial diversity and microbial carrying capacity and even distribution of species in phyllosphere as compared to single time spraying and control samples without spraying. Pareto Lorenz (PL) curve values obtained revealed that more than 80 % of bacterial diversity was represented by a specialized community present in phyllosphere. The bacterial dynamics in sprayed samples were higher than their unsprayed counterparts. From this study it was found that the indices of bacterial diversity, richness and evenness were found to be higher in phyllosphere receiving two sprays (on 15th and 30th) of Panchagavya as compared to single spray ad control samples.


Keywords: Phyllosphere, Panchagavya, Metagenomics, Diversity, Denaturing gradient gel electrophoresis (DGGE).
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How to cite this article:

Biradar Balasaheb Gunwantrao, C.R. Patil, Malik Ahmed Pasha. 2017. Bacterial Community Analysis of Soybean (Glycine max) Sprayed with Panchagavya Revealed by DGGE.Int.J.Curr.Microbiol.App.Sci. 6(4): 1207-1217. doi: https://doi.org/10.20546/ijcmas.2017.604.148