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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 544-551
DOI: https://doi.org/10.20546/ijcmas.2017.604.066


Isolation and Characterization of a Promising Plant Growth Promoting Rhizobacterium from Trichosanthes dioica Roxb. Rhizosphere
Satinath Das and Narayan C. Mandal*
Mycology and Plant Pathology Laboratory, Department of Botany, Visva-Bharati,Santiniketan 731235, West Bengal, India
*Corresponding author
Abstract:

A total number of 10 rhizosperic bacteria were isolated from root environment of some healthy plants of a drastic diseased field with massive blighted symptoms of Trichosanthes dioica of Akilpur of Hooghly district, West Bengal (22.94º E 88.12º N) where soil is alluvial in nature. Among the isolates, one strain TR9 showed remarkable plant growth promoting activity. Cell free culture filtrate of TR9 produced clear zones of inhibition (diameter ranging from 1.67cm to 2.67cm) against different plant pathogenic fungi viz. Colletotrichum acutatum, Alternaria alternata and Helminthosporium compactum. It is quite capable of lowering the pH of its environment and also can solubilise insoluble phosphate sources. Siderophore production by this strain gives a positive result. Biochemical characterization indicated the ability of the strain to utilize a broad range of carbon sources, catalase positivity and also to reduce the nitrate and nitrite. It is highly sensitive to 4-quinolone derivative antibiotics viz. ciprofloxacin, norfloxacin and resistant to beta lactams like ampicilin, cephalothin, cephalexin. Light compound microscopy showed it to be a Gram negative rod shaped one which was re established by scanning electron microscopy. All these characteristic features indicate the strain to be a species of Burkholderia.


Keywords: PGPR, Anti fungal, Scanning electron microscopy, Burkholderia.
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How to cite this article:

Satinath Das and Narayan C. Mandal. 2017. Isolation and Characterization of a Promising Plant Growth Promoting Rhizobacterium from Trichosanthes dioica Roxb. Rhizosphere.Int.J.Curr.Microbiol.App.Sci. 6(4): 544-551. doi: https://doi.org/10.20546/ijcmas.2017.604.066