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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
journals IJCMAS
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 836-844
DOI: https://doi.org/10.20546/ijcmas.2017.604.104


Chryseobacterium indologenes A Novel Root Nodule Endophyte in Vigna radiata
Archana Dhole*, Harsha Shelat and Deepak Panpatte
Anand Agricultural University, Anand, Gujarat, India-388110, India
*Corresponding author
Abstract:

Endophytic non rhizobial bacterial stain AM2 was isolated from root nodules of Vigna radiate. The bacterium was Gram-negative, yellow pigmented, nonspore forming, motile and oxidase positive. Phylogeny of 16S rRNA gene sequences revealed that the strain belonged to the genus Chryseobacterium, a member of family Flavobacteriaceae. Closest phylogenetic relatives to it were Chryseobacterium indologenes strain LMG8337 and Chryseobacterium indologenes strain NBRC 14944, respectively was showing 97% and 95% 16S rRNA gene sequence similarity. Strain showed presence of nifH gene of ~380 bp. It also exhibit nitrogen fixation ability around 19.55mg N g-1 of glucose consumed with indole acetic acid producing capability of 87.04 µg ml-1. However, phosphate solubilization and siderophore production was absent. 1-Aminocyclopropane-1-carboxylate deaminase activity was also present. In present study, to the best of our knowledge and literature surveyed endophytic non rhizobial C. indologenes AM2 isolated first time from root nodules of mungbean possessing plant growth promoting attributes.


Keywords: Endophytic non-rhizobial bacteria, Chryseobacterium indologenes, Plant growth promoting bacteria, Nitrogen fixation, nifH gene, Root nodule.
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How to cite this article:

Archana Dhole, Harsha Shelat and Deepak Panpatte. 2017. Chryseobacterium indologenes A Novel Root Nodule Endophyte in Vigna radiata.Int.J.Curr.Microbiol.App.Sci. 6(4): 836-844. doi: https://doi.org/10.20546/ijcmas.2017.604.104