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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 811-817
DOI: https://doi.org/10.20546/ijcmas.2017.604.102


Detection of Hepatozoan canis in Dogs from Namakkal Region of Tamilnadu by Conventional and Molecular Approach
Kalaivanan Murugesan1, Saravanan Subramanian1*, Palanivel Kondan Muthusamy1 and G. Ponnudurai2
1Department of Veterinary Preventive Medicine, Veterinary College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, (TANUVAS), Namakkal-2, India
2Department of Veterinary Parasitology, Veterinary College and Research Institute,Namakkal-2, India
*Corresponding author
Abstract:

A total of 150 dogs brought to Teaching Veterinary Clinical Complex (TCCC), Veterinary College and Research Institute, Namakkal, from September, 2015 to April, 2016 were screened for the haemoparasite, H. canis by blood smear examination and polymerase chain reaction which revealed a positivity of 0.66 and 5.33 per cent, respectively. The PCR was found to have a high sensitivity (100.0%) and specificity (91.27%) when compared with microscopic examination. The target 18S rRNA of H. canis identified was found to possess a 100.0 per cent homology with the H. canis of dogs in Hungary, Italy, Japan, North Africa, Pakistan, Portugal and Thailand.  Rhipicephalus sanguineus was the tick vector identified from the positive cases and the males (6.48%), cross breed dogs (10.0%) and the age group of >4 years (9.09%) were found to be highly susceptible.


Keywords: Prevalence,H. canis,  PCR, Microscopic examination, Phylogenetics, Sensitivity.   
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How to cite this article:

Kalaivanan Murugesan, Saravanan Subramanian, Palanivel Kondan Muthusamy and Ponnudurai, G. 2017. Detection of Hepatozoan canis in Dogs from Namakkal Region of Tamilnadu by Conventional and Molecular Approach.Int.J.Curr.Microbiol.App.Sci. 6(4): 811-817. doi: https://doi.org/10.20546/ijcmas.2017.604.102