National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A study was conducted to investigate in vitro pollen germination of four mango varieties, viz. Kesar, Alphonso, Mallika and Dudhpendo. Pollen grains were stored in three different media (n-hexane, paraffin oil and without media) under four different storage temperature (-20â°C, -4â°C, 4â°C and room temperature). The experiment was laid out in Completely Randomized Design (CRD) with factorial concept. Germination media having 10% sucrose + 100 ppm H₃BO₃ + 300 ppm Ca(NO₃)â‚‚ + 200 ppm MgSOâ‚„ + 100 ppm KNO₃ used for in vitro pollen germination for all the varieties. Variation due to different varieties, media and temperature was found significant. Among four varieties, maximum germination was recorded in Kesar during 7, 14, 21, 28, 35, 42 and 56 days of storage, whereas, in Mallika at 49 days of storage. In storage media, n-hexane found best for retaining more germination ability during all storage days. Room temperature storage of pollen showed no germination from 21 days of storage. Maximum pollen germination was recorded at -20â°C followed by -4â°C. On the basis of interaction effect of results obtained, it could be concluded that mango pollen grains of Kesar and Mallika could be stored successfully up to 56 days in n-hexane at -20â°C.
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