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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(4): 400-405
DOI: https://doi.org/10.20546/ijcmas.2017.604.045


Sensitivity Comparison of Immuno-Histochemical and Histopathological Approaches for Diagnosis of Rabies in Animals
Ajaz Ahmad1* and C.K. Singh2
1Department of Veterinary Pathology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana 141004, Punjab, India
2Animal Disease Research Centre, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana 141004, Punjab, India
*Corresponding author
Abstract:

To study the approach of immunohistochemistry (IHC) in comparison with study of pathomorphological alterations, for diagnosis of rabies, 50 brain samples from different animals like dog, cattle, buffalo and mongoose suspected for rabies were collected at post mortem hall. Out of the 50 animal brain samples tested, 30 (60%) were confirmed to be rabid by Fluorescent Antibody Technique (FAT). The 30 positive brain samples were subjected for comparison of histopathology and immunohistochemistry. Sensitivity of diagnosis of rabies by histopathology and IHC was 88.23% and 93.75% respectively. The specificity of diagnosis of rabies by histopathology and IHC was 100%each whereas the accuracy of diagnosis of rabies by histopathology and IHC was 92.59%and 96.15% respectively. The immunohistochemistry is more sensitive than histopathology for diagnosis of rabies in formalin fixed brain samples. It is, therefore, recommended to supplement histopathology with IHC as an alternative diagnostic technique especially in the laboratories that resort to histopathology for diagnosis of rabies, in absence of facilities for immunofluorescence.


Keywords: Fluorescent antibody test, Histopathology, Immuno  histochemistry, Rabies.
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How to cite this article:

Ajaz Ahmad and Singh, C.K. 2017. Sensitivity comparison of immuno-histochemical andhistopathologicalapproaches for diagnosis of Rabies in animalsInt.J.Curr.Microbiol.App.Sci. 6(4): 400-405. doi: https://doi.org/10.20546/ijcmas.2017.604.045