Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
journals IJCMAS
See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(3): 2430-2437
DOI: https://doi.org/10.20546/ijcmas.2017.603.277


Molecular Diagnosis of Human Metapneumovirus Infection among Egyptian Infants with Acute Bronchiolitis
Gamal El-Din El-Sawaf1, Maged Eissa2, Abeer Ghazal1, Dalia Metwally1, Rania Abozahra3* and Walid Rafla4
1Department of Microbiology, Medical Research Institute,Alexandria University, Alexandria, Egypt
2Department of Pediatrics, Faculty of Medicine, Alexandria University, Alexandria, Egypt
3Department of Microbiology and Immunology, Faculty of Pharmacy,Damanhour University, Damanhour, Egypt
4Department of Microbiology and Biotechnology, Faculty of Science,Alexandria University, Alexandria, Egypt
*Corresponding author
Abstract:

Lower respiratory tract infections are one of the leading causes of morbidity and mortality in children worldwide. Several viruses can cause bronchiolitis. Respiratory syncytial virus (RSV), parainfluenza viruses (PIV), influenza type A, adenovirus and human metapneumovirus (HMPV) are some of the common viruses found in hospital studies.  This study aimed to study the molecular diagnosis of HPMV infections by using a real-time, reverse-transcriptase PCR assay and the evaluation of its prevalence among Egyptian infants clinically diagnosed with acute viral bronchiolitis. Also to detect other common respiratory viruses and determine the presence of coinfections of HPMV and other respiratory viruses, we tested the presence of common respiratory tract viruses by using immunofluorescence respiratory screen test in 50 Egyptian infants diagnosed with acute bronchitis and also screened the tested samples for the presence of HMPV by using real-time RT PCR. Our results revealed that 16% of the cases were positive for HMPV, 76% were positive for the other tested common respiratory tract viruses and 12% showed coinfection.


Keywords: Human Metapneumovirus, Bronchiolitis, RT PCR, Infants.
Download this article as Download

How to cite this article:

Gamal El-Din El-Sawaf, Maged Eissa, Abeer Ghazal, Dalia Metwally, Rania Abozahra and Walid Rafla. 2017. Molecular Diagnosis of Human Metapneumovirus Infection Among Egyptian Infants With Acute Bronchiolitis.Int.J.Curr.Microbiol.App.Sci. 6(3): 2430-2437. doi: https://doi.org/10.20546/ijcmas.2017.603.277