Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
journals IJCMAS
See Guidelines to Authors
Current Issues

Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(3): 2349-2362
DOI: https://doi.org/10.20546/ijcmas.2017.603.269


A Novel Alkaline, Highly Thermostable and Oxidant Resistant Carboxymethyl Cellulase (Cmcase) Produced by Thermophilic Bacillus sonorensis CY-3
Yasemin Caf1,2* and Burhan Arikan1
1Biotechnology Department, Institute of Basic and Applied Sciences,Cukurova University, Turkey
2Molecular Biology and Genetics Department, Institute of Basic and Applied Sciences,
Avrasya University, Turkey
*Corresponding author
Abstract:

The bacterial strain a thermophilic carboxymethyl cellulase-producing was screened from biocompost. The strain was identified as Bacillus sonorensis CY-3 according to morphological, biochemical and molecular analysis, then it was optimized for the production of carboxymethyl cellulase (CMCase). Enzyme was optimally produced in a Luria Broth (LB) medium containing carboxymethyl cellulose (CMC) at pH 9.0 and 55ºC. Two bands were found with molecular mass 80 kDa and 72 kDa by use SDS-PAGE and the Vmax and Km were measured 380.19 U/ml and 5.82 mg/ml, respectively. The partially purified enzyme has showed optimal activity at pH 9.0 and 100°C while it was stabled from pH 6.0 to 13.0 with more than 65% activity. It was found to have the properties of enzyme highly thermostability, pH stability, and stability in the presence of some additives that made potentially useful in textile, laundry, and other industrial applications.


Keywords: Bacillus sonorensis, Thermostable enzyme, Cellulase activity, Oxidant residance, Carboxymethylcellulose.
Download this article as Download

How to cite this article:

Yasemin Caf and Burhan Arikan. 2017. A Novel Alkaline, Highly Thermostable and Oxidant Resistant Carboxymethyl Cellulase (Cmcase) Produced by Thermophilic Bacillus sonorensis CY-3.Int.J.Curr.Microbiol.App.Sci. 6(3): 2349-2362. doi: https://doi.org/10.20546/ijcmas.2017.603.269