National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
This study was designed to evaluate the antiulcer activity of Commiphora africana stem-bark extracts using ethanol induction model in laboratory rats and to identify phytochemical constituents of the extracts responsible for the observed activity. C. africana stem-bark was extracted with n-hexane, ethyl acetate and methanol gradient wise in a soxhlet apparatus at 50ºC. The extracts were subjected to qualitative phytochemical analysis, toxicity experiment and anti- ulcer evaluation using ethanol – induced gastric ulcer in laboratory rats. A standard anti-ulcer agent, omeprazole was used as reference standard. The data were analyzed by one-way Analysis of Variance with significant level at (p<0.05). The percentage yield from the gradient extraction of C. africana stem-bark showed methanol to be the highest. Thin Layer Chromatographic analysis visualized with specific reagents confirmed the presence of steroids/triterpenes, phenolic compounds and flavonoids in the stem-bark of C. africana. LD50 was above 5000 mg/kg and did not cause mortality in all the tested rats. Ethanol triggered severe gastric ulcers with mean ulcer index (12.8 ± 0.97 mm) and pretreatment with C. africana stem-bark n-hexane, ethyl acetate and methanol extracts at (250, 500 and 1000 mg/kg) and Omeprazole (20 mg/kg) produced a significant (p<0.05) dose dependent anti-ulcer activity with increase in percentage ulcer inhibition of (9.38%, 43.75% and 57.81%) for CAHE, (59.38%, 68.75% and 85.94%) for CAEE and (65.63%, 73.44% and 90.63%) for CAME respectively. Omeprazole had 68.75% ulcer inhibition. This study demonstrated that C. africana has anti-ulcer potential and it justified the traditional uses of the stem-bark in ulcer treatment.
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