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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(3): 1897-1905
DOI: https://doi.org/10.20546/ijcmas.2017.603.215


Effect of Sunlight and UV Light against DpNPV  (Nuclear Polyhedrosis Virus) Formulation on Larval Mortality of Mulberry Leaf Webber, Diaphania pulverulentalis Hampson
S. Prabhu1* and C.A. Mahalingam2
1Department of Agricultural Entomology, RVS Padmavathy College of Horticulture (Affiliated to Tamil Nadu Agricultural University, Coimbatore), Sempatti- 624 707, India
2 Department of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore- 641 003, India
*Corresponding author
Abstract:

Effect of sunlight and UV light on the inactivation of nuclear polyhedrosis virus to Diaphania pulverulentalis (Hampson). Larvae were studied under laboratory conditions. The larval mortality was significantly maximum (76.66%) and (86.65%) when DpNPV suspension and formulation was exposed to sunlight for 1 hour respectively. Minimum mortality of (48.33%) and (61.66%) of DpNPV suspension and formulation were observed when it was exposed to sunlight for 5 hours which indicating reverse relationship between the exposure duration to sunlight and larval mortality. Inactivation of DpNPV by UV light was demonstrated by the decreased mortality rate in larval groups inoculated with irradiated virus compared to those fed on non- radiated virus i,e., control. Significant difference in larval mortality was observed when DpNPV was exposed to 5, 10, 15, 30 and 60 minutes to UV light, (88.33, 85.00, 80.00, 66.66 and 53.33 % mortality respectively). Whereas in formulated DpNPV, the rate of larval mortality recorded was 93.33, 90.00, 86.65, 71.66 and 66.66 per cent when exposed to 5, 10, 15, 30 and 60 minutes to UV light respectively. The inactivation of virus was directly related to the period of exposure to UV radiation. This finding indicated a loss in pathogenicity and the number of days to death was higher and the total mortality was lower from exposed virus than from unexposed virus.


Keywords: Mulberry, Diaphania pulverulentalis, NPV, Sunlight,UV light.
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How to cite this article:

Prabhu, S. and Mahalingam, C.A. 2017. Effect of Sunlight and UV Light against DpNPV (Nuclear Polyhedrosis Virus) Formulation on Larval Mortality of Mulberry Leaf Webber, Diaphania pulverulentalis Hampson.Int.J.Curr.Microbiol.App.Sci. 6(3): 1897-1905. doi: https://doi.org/10.20546/ijcmas.2017.603.215