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An experiment was conducted in cemented pot in “screen house” of the Department of Soil Science and Agricultural Chemistry, Chandra Shekhar Azad University of Agriculture and Technology, Kanpur during Rabi season 2011-2012, to assess the response of inoculation of rhizobium and application of different doses of iron on nutrient uptake in chickpea. Treatments consisted four levels of iron i.e. 0, 2.5, 5.0 and 7.5 ppm and two levels of microbial inoculation i.e. un-inoculation and inoculation of rhizobium. The treatments were replicated four times in completely randomized design (factorial). The results indicated that among the iron levels, 5.0 ppm showed better expression on uptakeof nitrogen (657.21 mg pot-1, 192.25 mg pot-1,), phosphorus (111.40 mg pot-1, 67.48 mg pot-1), iron (1.520 mg pot-1, 2.753 mg pot-1) and consequently recorded significantly higher in comparison to 0, 2.5 and 7.5 ppm levels. The seed treatment of chickpea with rhizobium culture significantly increased the nutrient uptake in grain and stover.