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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(3): 1339-1344
DOI: https://doi.org/10.20546/ijcmas.2017.603.155


Phytochemical Analysis and Antibacterial Activity of Annona muricata (Laxman phal) against ESBLs Producers (Escherichia coli and Klebsiella pneumoniae)
N. Kamath1*, Rita Swaminathan2 and Neetin Desai3
1Department of Microbiology, World College of Medical Sciences and Research and Hospital, Jhajjar, Harayana, India
2Department of Microbiology, D.Y. Patil School of Medicine and Hospital,
Nerul, Navi Mumbai, India
3Department of Biotechnology, Amity University, Panvel, Navi Mumbai, India
*Corresponding author
Abstract:

The use of plant products as medicine has emerged from traditional days to modern times of medicine in order to improve the quality life of the patients worldwide. Nature-derived medicines are considered safer with less or no side effects. The antibacterial effect of the leaves of Annona muricata was evaluated on bacterial strains of ESBLs: Escherichia coli and Klebsiella pneumoniae are tested which were isolated from D.Y. Patil Hospital, Nerul, NaviMumbai. Standard strain of Escherichia coli ATCC 25922 has been tested for cephalosporin (Ceftazidime- zone of inhibition: 31mm) and Imipenem (zone of inhibition: 25 mm) and is found to be sensitive. The solvents used for the extraction of plants were water (aqueous) and ethanol. The antibacterial activity was done by using Kirby-Bauer disc diffusion method. Both extracts showed antibacterial properties but the ethanolic extract was more effective as it inhibited a wide range of organisms at varying concentrations. The most susceptible Gram-negative bacteria was Escherichia coli. The significant antibacterial activity of plant extracts was compared with the standard antibiotic, Imipenem. The results obtained in the present study suggest that Annona muricata can be used in treating diseases caused by the test organisms and or can be used along with the antibiotics having synergistic activity.


Keywords: Laxman phal, Kirby-Bauer technique, Ethanol extract and aqueous extract, ESBL-extendended spectrum betalcatmases.
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How to cite this article:

Kamath, N., Rita Swaminathan and Neetin Desai. 2017. Phytochemical analysis and antibacterial activity of Annona muricata (Laxman phal) against ESBLs producers (Escherichia coli and Klebsiella pneumoniae).Int.J.Curr.Microbiol.App.Sci. 6(3): 1339-1344. doi: https://doi.org/10.20546/ijcmas.2017.603.155