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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2017.6(3): 718-724
DOI: https://doi.org/10.20546/ijcmas.2017.603.083


Comparison of Wet Mount Examination with Giemsa Staining and Fluorescent Staining for Detection of Trichomonas vaginalis in Clinically Suspected Cases of Vulvovaginitis
Vartika Paliwal1, Arti Jain1, Avinash Laghawe1, Kaore Navinchandraand Tukaram Prabhu1
1People’s College of Medical Sciences & Research Center, Bhanpur Road, Bhopal -462037
 Madhya Pradesh, India
2Raipur Institute of Medical Sciences, Gram Godhi, Bhansoj Road, Raipur, (C.G), India
*Corresponding author
Abstract:

Trichomonas vaginalis is an important cause of the premature rupture of membranes, premature delivery, pelvic inflammatory disease, urethritis, and chronic prostatitis. Wet mount is the most widely used method for the detection of T. vaginalis in clinically suspected cases of vulvovaginitis. The purpose of this Study was to compare various microscopic techniques for detection of T. vaginalis like wet mount examination, Giemsa and Acridine orange staining. The Cross-sectional Analytical study was conducted in the Department of Microbiology, attached to a Tertiary Care Hospital in Central India after obtaining ethical clearance over a time period of two months. Out of 30 cases, only two (6.66%) were detected positive for Trichomoniasis. All the 30 vaginal samples (cases) tested for detection of T. vaginalis were found to be negative by Wet mount examination. Whereas, Giemsa staining and Acridine orange staining were positive in 1 (3.33%) and 2 (6.66%) case respectively. Present study highlights that Acridine orange staining was found to be the best microscopic method when compared with wet mount examination and Giemsa staining. This will also provide rapid screening of vaginal smears in patients suffering from vaginal discharge and thus, help in early diagnosis and prompt treatment of patients.


Keywords: Trichomonas vaginalis, Wet mount examination, Giemsa staining, Acridine orange staining.
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How to cite this article:

Vartika Paliwal, Arti Jain, Avinash Laghawe, Kaore Navinchandra, Tukaram Prabhu. 2017. Comparison of Wet Mount Examination with Giemsa Staining and Fluorescent Staining for Detection of Trichomonas vaginalis in Clinically Suspected Cases of Vulvovaginitis.Int.J.Curr.Microbiol.App.Sci. 6(3): 718-724. doi: https://doi.org/10.20546/ijcmas.2017.603.083