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A field experiment was conducted to examine the effect of early post-emergence herbicide Imazethapyr 10% SL on soil enzyme activity (Dehydrogenase, Urease and Phosphatase) and nutrient uptake by weeds and greengram at Main Agricultural Research Station, Dharwad during kharif 2014. The treatments comprised of Imazethapyr 50g, Imazethapyr 62.5g, Imazethapyr 75g, Imazethapyr 50g with adjuvant, Imazethapyr 62.5g with adjuvant, Imazethapyr 75g with adjuvant, Quizalofop-ethyl 1000 ml, standard check (2IC + 2HW), weedy check and weed free check and was laid out in Randomized Complete Block Design with three replications. Weed free check (T10) recorded significantly higher dehydrogenase, urease and phosphatase activity recorded at seven & 15 days after spraying compared to all other treatments but was on par with T8. Among the different doses, higher dose of Imazethapyr (75 g a.i. ha-1 with adjuvant) recorded lower dehydrogenase, urease and phosphatase activity of soil at seven and 15 days after spraying. Weed free check (T10) recorded significantly higher uptake of nutrients (NPK) by greengram compared to all other treatments but was on par with T8. Among the different doses, higher dose of Imazethapyr (75 g a.i. ha-1 with adjuvant) recorded higher uptake of nutrients (NPK) by greengram. Nutrients (NPK) uptake by weeds was higher in weedy check, but significantly lower in weed free, standard check and Imazethapyr treated plots.