National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
An attempt was made to isolate and characterize rhizobacteria from rhizosphere soils of different crops grown in Vijayapur, Kalaburgi and Bagalkot districts of Northern Karnataka. Ten PGPR isolates and reference strain were subjected for functional characterization viz., IAA and GA production, P and Zn solubilization. Among the ten native rhizobacterial isolates, 8 isolates and reference strain produced IAA and GA. The amount of IAA and GA produced by the different rhizobacterial isolates ranged from 10.78 to 20.17 µg/ml of broth and 0.36 to 2.69 µg/ml of broth respectively. The diameter of P and Zn solubilization by the rhizobacterial isolates ranged from 3 to 7.5 mm and 2.0 to 6.0 mm after five days of inoculation respectively. These isolates were further subjected for morphological and biochemical characterization. The isolates exhibited wide variations with respect to colony morphology. Except one isolate (PG-189), other isolates found to have rod shaped cells. Except PG-152 and PG-186, other isolates and reference strain were found to be gram negative. Endospore formation was observed only in the isolate PG-152. With respect to biochemical characterization, all the isolates and reference strain were positive for oxidase and ammonia production tests and negative for indole production and lactose fermentation.
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