Growth of Botryodiplodia theobromae an Incitant of Longitudinal Splitting of Bark and Wood Disease in Acidlime (Citrus aurantifolia Swingle) as Influenced by pH Levels, Temperature and Growth Media

T. Gouri Sankar; V. Gopi; Y. Hema Bharathi; K. Gopal; L.Mukunda Lakshmi

doi:http://dx.doi.org/10.20546/ijcmas.2016.512.086

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Original Research Articles Volume : 5, Issue:12, December, 2016

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Int.J.Curr.Microbiol.App.Sci.2016.5(12): 756-764

DOI: http://dx.doi.org/10.20546/ijcmas.2016.512.086

Growth of Botryodiplodia theobromae an Incitant of Longitudinal Splitting of Bark and Wood Disease in Acidlime (Citrus aurantifolia Swingle) as Influenced by pH Levels, Temperature and Growth Media

T. Gouri Sankar¹, V. Gopi¹, Y. Hema Bharathi¹, K. Gopal^2* and L.Mukunda Lakshmi¹

¹Molecular Diagnosis Laboratory, Citrus Research Station, Dr. Y.S.R. Horticultural University,
Tirupati – 517 502, Andhra Pradesh, India
²College of Horticulture, Dr. Y.S.R. Horticultural University, Anantharajupeta – 516 105, Andhra Pradesh, India

^*Corresponding author

Abstract:

Studies on effect of pH levels, temperature and growth media on growth of Botryodiplodia theobromae causing longitudinal splitting of bark and wood disease on acid lime revealed that higher dry mycelial weight of the fungus was obtained at 25 to 30⁰C which was considered as optimum for the vegetative growth. Among the different media tested Potato Dextrose Broth (PDB) supported for maximum dry mycelial weight followed by Richards Broth (RB), Czapek Dox Broth (CPB) and Czapek Dox Broth (CDB) medium. Among the different media used PDB gave maximum dry mycelial weight at pH ranged from 5.0 to 6.0 followed by CPB, CDB and RB media.

Keywords: Botryodiplodia theobromae, Longitudinal Splitting of Bark and Wood Disease (LSPWD), Growth.

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How to cite this article:

Gouri Sankar, T., V. Gopi, Y. Hema Bharathi, K. Gopal and L. Mukunda Lakshmi. 2016. Growth of Botryodiplodia theobromae an Incitant of Longitudinal Splitting of Bark and Wood Disease in Acidlime (Citrus aurantifolia Swingle) as Influenced by pH Levels, Temperature and Growth MediaInt.J.Curr.Microbiol.App.Sci. 5(12): 756-764. doi: http://dx.doi.org/10.20546/ijcmas.2016.512.086

Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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Original Research Articles Volume : 5, Issue:12, December, 2016

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