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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:12, December, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(12): 151-160
DOI: http://dx.doi.org/10.20546/ijcmas.2016.512.017


Mitigation of Toxic Effects of Sodium Arsenate on Germination, Seedling Growth and Amylolytic Enzyme of Mungbean Seedlings with Macronutrients, Micronutrients and Organic Acids
Arpita Swarnakar*
Bangabasi College, University of Calcutta, West Bengal-700009, India
*Corresponding author
Abstract:

Effect of sodium arsenate (Na2HAsO4.7H2O) on the growth of mungbean seedlings was investigated and it was found to have drastic effects on the germination and growth of mungbean (Vigna radiata (L.) Wilczek cv. B-105) seedlings. Percentage of seed germination was found to decrease  significantly with increasing concentrations (5μM, 10μM and 20μM)  of  arsenate. Reduction in seedling length - shoot length, root length, area of primary leaves, fresh weight and dry weight was also observed. Similar decreasing trend was also recorded in case of amylolytic enzyme activity i.e. amylase activity of the seedlings. However, reduction in seedling growth inhibition was achieved by pretreating the seeds with macronutrients (KH2PO4, NaH2PO4.2H2O, K2SO4, CaCl2.2H2O, MgSO4.7H2O at 10mM concentration), micronutrients (ZnSO4, CuSO4, Na2MoO4.2H2O, MnCl2.4H2O, CoCl2.6H2O, FeSO4.7H2O at 1mM concentration) and organic acids (pyruvate, citrate, malate, fumarate, succinate at 10mM concentration); best results were shown by KH2PO4, NaH2PO4, CoCl2 and Pyruvate from above mentioned three respective groups. These vital compounds used  in  the  present   study helped in the  mitigation of arsenate induced growth inhibition to different degrees. Growth enhancement of seedlings caused by these two phosphate salts with different cations indicates that anion phosphate and not cations (Na and K) is responsible for the beneficial effect.


Keywords: Sodium arsenate, macronutrients, micronutrients, organic acids, mitigation,Vigna  radiata.

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How to cite this article:

Arpita Swarnakar. 2016. Mitigation of Toxic Effects of Sodium Arsenate on Germination, Seedling Growth and Amylolytic Enzyme of Mungbean Seedlings with Macronutrients, Micronutrients and Organic Acids.Int.J.Curr.Microbiol.App.Sci. 5(12): 151-160. doi: http://dx.doi.org/10.20546/ijcmas.2016.512.017
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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