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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
journals IJCMAS
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(12): 141-150
DOI: http://dx.doi.org/10.20546/ijcmas.2016.512.016


Study of Ligninolytic Bacteria Isolation and Characterization from Kuthrel Agro Field of Bhilai-Durg Region
Sabiha Naz*
Department of Biotechnology, Bhilai Mahila Mahavidyalaya, Hospital sector, Bhilai, India
*Corresponding author
Abstract:

After cellulose; lignin is the most abundant renewable carbon source on Earth and very resistant to degradation. In present study isolation and characterization of ligninolytic bacteria were done from Kuthrel agro-fields of Bhilai-Durg regions. Isolation of microbes was done from both mixed and black soil samples by Hungate method. Result shown that four types of bacterial colonies (2-MS, 2-BS) were isolated from agro field. Out of these only 2 types of colonies were shown potential of lignin degradation. The Morphological, biochemical characteristics of the isolates were identified (H)-Bacillus sp. and (h) – Streptomyces sp. with reference to Bergey’s Manual of Determinative Bacteriology. These identified isolates Bacillus sps. and Streptomyces sps. were found to have the potential to tolerate high concentrations of kraft lignin and produced all three main ligninolytic enzymes (lignin peroxidase, manganese peroxidase, and laccase). These enzymes were quantified in 2 bacterial strains evaluated at pH 7.4 (LiP = veratryl alcohol-guaiacol; MnP = lactate-guaiacol-Mn+2; laccase = ABTS-catalase). From these, two strains (Bacillus species and Streptomyces species) showed the most activity for Li peroxidase strains Bacillus species and Streptomyces species also had the greater activity for Mn peroxidase and Laccase. This result concluded that both kinds of strains were able to degrade lignin substrate and have specific advantages for the depolymerization of the modified lignin residues typically encountered in waste streams from the pulping or 2nd generation biofuel/biobased chemicals industry.


Keywords: Lignin,Laccase,Manganese peroxidase,Lignin peroxidase,Bacillus sps., Streptomyces sps.
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How to cite this article:

Sabiha Naz. 2016. Study of Ligninolytic Bacteria Isolation and Characterization from Kuthrel Agro Field of Bhilai-Durg Region.Int.J.Curr.Microbiol.App.Sci. 5(12): 141-150. doi: http://dx.doi.org/10.20546/ijcmas.2016.512.016