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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(12): 131-140
DOI: http://dx.doi.org/10.20546/ijcmas.2016.512.015


Antibacterial Activity of Silver nanoparticles and Pomegranate (Punica granatum L.) Extracts
Asma G. Oraibi*, Rana A. Mohsien, Widad J. Atea, Haider N. Yahia and Lujian Raheem
 College of applied biotechnology, Al-Nahrian university, Baghdad, Iraq
*Corresponding author
Abstract:

Water and ethanol extracts of Punica granatum peels were prepared at the concentration 0.5 and 1.0mg/10ml, silver nanoparticles (4000mg/l) prepared at the same concentrations (0.5 and 1.0mg/10ml). Bacterial samples used in the experiment consisted of two gram-negative bacteria (Escherichia coli and Pseudomonas erogenous) and two gram-positive bacteria (Staphylococcus aurous and Streptococcus pyogenes). All bacterial types were treated either with plant extracts alone or mixed with the same concentrations of silver nanoparticles. Results showed that there was a significant decrease recorded in the mean inhibition zone at 0.5mg/10ml peel water extract (B) (27.5mm), 0.5mg/10ml peel ethanol extract (C) (25.5mm) and 1.0mg/10ml peel ethanol extract (H) (27.7mm) in comparison to the control (A) (32.5mm). According to the type of bacteria the highest mean inhibition zone observed in Staphylococcus aurous bacteria (34.8mm) and the lowest mean obtained in Pseudomonas erogenous bacteria (16.3mm). The interaction between the type of bacteria and treatment types indicated that the highest mean inhibition zone recorded in 1:1 (v/v) of  0.5mg/10ml silver nanoparticles and ethanol peel extract (F), and 1:1 (v/v) of  1.0mg/10ml silver nanoparticles and ethanol peel extract (K) with Staphylococcus aurous bacteria (39.0mm). While there was a significant reduction recognized in the mean inhibition zone with other types of bacterial strains used in the experiment in comparisons to the control (250mg/10ml cefotaxime).


Keywords: Punica granatum, antibacterial activity,silver nanoparticles. 
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How to cite this article:

Asma G. Oraibi, Rana A. Mohsien, Widad J. Atea, Haider N. Yahia and Lujian Raheem. 2016. Antibacterial Activity of Silver nanoparticles and Pomegranate (Punica granatum L.) Extracts.Int.J.Curr.Microbiol.App.Sci. 5(12): 131-140. doi: http://dx.doi.org/10.20546/ijcmas.2016.512.015