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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
journals IJCMAS
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Original Research Articles

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(12): 106-111
DOI: http://dx.doi.org/10.20546/ijcmas.2016.512.012


Sero-Prevalence of Dengue Infection by Rapid Immunochromatographic Assay from Tertiary Care Medical College Hospital in Coimbatore, India
Gnana Prakash1, R. Someshwaran2* and Anbu N. Aravazhi3
Department of Microbiology, Karpagam Faculty of Medical Sciences and Research, Othakkalmandapam, Coimbatore-641032, India
*Corresponding author
Abstract:

Dengue infection is an acute febrile endemic viral disease mostly prevalent in the tropical countries. The serological diagnosis of primary dengue infection is made by detection of NS1 antigen which is important for early (Day 1-7 of fever) and accurate diagnosis of dengue. This study is aimed to evaluate the prevalence of dengue infections by using Rapid Immuno-Chromatographic card Test detecting NS1 Antigen, Anti-IgM and Anti-IgG DENV antibodies among clinically suspected Dengue virus infections from patients attending a tertiary care hospital. This study was conducted in the form of Cross sectional study where total of 1469 serum samples received in serology section with working diagnosis of Dengue fever or other viral illnesses were subjected to Dengue virus screening for NS1, Anti-IgM and Anti-IgG Antibodies in serum was done by using Rapid Immuno-chromatographic Card Tests. Out of 452 clinically diagnosed dengue fever cases, 32 (7.08%) were positive for Dengue NS1 antigen alone. And the prevalence of NS1 Antigenemia was found to be 7.08% that is 32 out of 452 clinically diagnosed Dengue fever cases in this current study. The prevalence of Acute Dengue virus infections was found to be 8.85%. Rapid Immuno chromatographic card tests are simple, feasible, cost-effective, less time consuming, with less labor involved, where sensitivity and specificity are good, but this card tests are only a screening test and so it cannot be used as a stand-alone test for diagnostic confirmation. , but it could be supplemented with another diagnostic assays like ELISA for Dengue IgM and IgM, Indirect Immuno fluorescence and Polymerase Chain Reaction for appropriate diagnosis of DENV infection.


Keywords: NS1 antigenemia, IgM,IgG antibody , Acute Dengue,  Complications, RCT,
Screening.
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How to cite this article:

Gnana Prakash, K., R. Someshwaran and Anbu N. Aravazhi. 2016. Sero-Prevalence of Dengue Infection by Rapid Immunochromatographic Assay from Tertiary Care Medical College Hospital in Coimbatore, India.Int.J.Curr.Microbiol.App.Sci. 5(12): 106-111. doi: http://dx.doi.org/10.20546/ijcmas.2016.512.012