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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
journals IJCMAS
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(12): 65-71
DOI: http://dx.doi.org/10.20546/ijcmas.2016.512.007


Characterization of Escherichia coli from Stool Samples in Children with Diarrhea and their Antibiotics Resistance Pattern
Taiyaba1*, Farhat Tahira1 and Mohammad Azam2
1Department of Microbiology, Career Institute of Medical Sciences
and Hospital Lucknow, India
 2CMS, Department of Community Medicine, Career Institute of Medical Sciences and Hospital Lucknow, India
*Corresponding author
Abstract:

Diarrheal diseases are a major cause of morbidity and mortality in developing countries. In the past decades several new enteric pathogens, including bacterial, viral, and parasitic agents have been described. Escherichia coli (Esch. coli) is one of the leading cause of acute diarrhea in developing countries in children under 5 years old, with significant morbidity and mortality. The present study was carried out in the Department of Microbiology Mayo Institute of Medical Sciences and Research Lucknow. The study group included 81 children suffering from diarrhoea attending the inpatient or outpatient sections of the Department of Paediatrics. Among the bacterial pathogens detected, Esch. coli was the commonest. In the present study major symptoms reported were diarrhea, fever and vomiting. Examination of stool samples showed that 32 (39.5%) children harbored one or more of the Escherichia coli pathotypes. Out of those 32 who were subjected to serotyping  9 (28.12%) were untypable and 5 (15.62%) were rough strains.  Of the typable strains, Enteropathogenic E.coli (EPEC) accounted for 3.12%, Enterotoxicogenic E.coli (ETEC) accounted for 12.5%, Enterohaemorragic (EHEC) accounted for 3.12 %. Of the ETEC strains, serogroups O173 (1), O11 (1), O15 (1) and O8 (1) were found.  The EPEC strain found was O86 and EHEC was serogroup O172.  Among the other serogroups which comprised of 37.5% strains, O161 (3) was predominant. Antibiotic resistance was documented for Ampicillin (100%).


Keywords: Escherichia coli, Diarrhea, Pathotype, Antimicrobial susceptibility.
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How to cite this article:

Taiyaba, Farhat Tahira and Mohammad Azam. 2016. Characterization of Escherichia coli from Stool Samples in Children with Diarrhea and their Antibiotics Resistance Pattern.Int.J.Curr.Microbiol.App.Sci. 5(12): 65-71. doi: http://dx.doi.org/10.20546/ijcmas.2016.512.007