|PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
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Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38
Studies on molecular mechanism of Trichoderma viride in the pathogenesis action on nematode eggs mostly focus on the transcriptional activity of selected genes. However, studies investigating the exact role of a specific gene induction in T. viride on nematode egg parasitism are scant. The molecular interface between T. viride and Meloidogyne incognita eggs was investigated through the qPCR gene expression. The resultant gene chi18-5 of T. viride played a vital role in the induction of mycosis in the nematode eggs. Real-time PCR revealed that chi18-5 started up regulating at 2 hours post-inoculation (hpi) with maximum expression at 5 hpi, and the up regulation of the gene gradually decreased until 19 hpi. The chitinolytic enzyme chi18-5 plays a major role in egg parasitism in transcriptional activity in T.viride was characterized. Our present study proved that chi18-5 encodes one of the lytic enzymes required by T. viride to parasitise nematode eggs.