National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A soil survey was carried out during 2016-2017 in four predominantly Sugarcane growing districts of Telangana state, viz., Sangareddy, Kamareddy, Nizamabad and Khammam, for the presence of entomopathogenic nematodes (EPNs) from Sugarcane ecosystem. The soil samples collected from Kamareddy district showed the highest occurrence of EPNs in 33.33% of the soil samples, followed by Sangareddy (23.33%), Khammam (16.67%) and Nizamabad (5.55%) districts. The isolates of EPNs were utilized for molecular characterization and identification. The genomic DNA of EPN isolates was extracted and 18S and 28S ITS rDNA region of genomic DNA was amplified by PCR technique using a universal primer. The molecular studies shown the occurrence of Heterorhabditis indica in 41.18% of the soil samples, followed by Heterorhabditidoides sp. in 23.53% and Heterorhabditis bacteriophora in 17.65% of the soil samples. The results concluded that H. indica was dominant EPN species occurring in Sugarcane ecosystem. The new EPN species Heterorhabditidoides spp. was reported for the first time in India.
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