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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here
ICV 2022: 95.28
Index Copernicus ICI Journals Master List 2022 - IJCMAS--ICV 2022: 95.28
For more details click here
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Original Research Articles                      Volume : 5, Issue:11, November, 2016
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
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Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38
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Int.J.Curr.Microbiol.App.Sci.2016.5(11): 702-706
DOI: http://dx.doi.org/10.20546/ijcmas.2016.511.081


Importance of EDTA in the Detection of Metallo Beta Lactamase from Imipenem Resistant Gram Negative Bacilli
P. Kanchanadevi1* and S. Chandra Sekaran2
1CSI College of Dental Sciences and Research, Eastveli Street, Madurai, India
2NIMRA Institute of Medical Sciences, Jupidi village, Ibrahimpatnam, Mandal, Krishna DT, Vijaywada, Andhrapradesh, India
*Corresponding author
Abstract:

Gram negative Bacilli isolates resistant to imipenem were screened phenotypically for  Metallo Beta Lactamase( MBL) production by EDTA Disc Synergy (EDS) method and MIC reduction test. EDTA, a chelating agent which inhibits the production of MBL and hence incorporation of EDTA to the resistant strains, results in the zone formation. In this study, Minimal Inhibitory Concentration for Imipenem was also estimated and the incorporation of EDTA to the drug dilution plates differentiated all MBL producing Gram negative Bacilli.


Keywords: MBL(Metallo Beta Lactamase),MIC   EDTA  (Ethylene DiamineTetra Aceticacid).

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How to cite this article:

Kanchanadevi, P., and S. Chandra Sekaran. 2016. Importance of EDTA in the Detection of Metallo Beta Lactamase from Imipenem Resistant Gram Negative Bacilli.Int.J.Curr.Microbiol.App.Sci. 5(11): 702-706. doi: http://dx.doi.org/10.20546/ijcmas.2016.511.081
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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