National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
A set of 45 polymorphic microsatellite markers, representing markers for almost each chromosome, was used for the assessment of genetic diversity in 35 genotypes of bread wheat. A total of 127 alleles were detected which ranged from 2 to 6 with an average allele number of 2.82 per locus. The PIC value of the 45 SSRs ranged from 0.08 to 0.50 with an average PIC of 0.30. Resolving power and Marker index of all 45 markers varied from 0.17 to 2.82 and 0.16 to 1.52, respectively. Genome wise comparison of diversity recorded maximum genomic diversity in genome A with highest number of alleles per locus (2.95), PIC (0.31), RP (1.33) and MI (0.90). A comparative study on the basis of homeologous chromosomes revealed homeologous chromosome 1 had highest number of alleles per locus (3.0), PIC value (0.32), RP (1.40) and MI (0.92). The homeologous chromosomes 5 and 7 were found to be most conserved with low PIC, RP and MI. The dendrogram, prepared on the basis of similarity matrix using the UPGMA algorithm, delineated the all genotypes into three clusters with 13, 11 and 11 genotypes in cluster I, II and III, respectively. The results demonstrate the utility of microsatellite markers for detecting polymorphism and for estimating genetic diversity in wheat.
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