National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The caprine milk proteins are considered as power house of bioactive peptides, these bioactive peptides may be released in enzymatic hydrolysis(Trypsin 10 mg of enzyme/5 g of protein) at pH 8.0 and temperature 400 C was of casein fractions was carried out at an enzyme- substrate (E: S) ratio of 1:25. Enzymatic hydrolysis of casein fractions was carried out at an enzyme- substrate (E: S) ratio of 1:25. The milk protein from goat milk was extracted by using acid precipitation method and purified using urea fractionation. Fractionation of whole casein from 6.6 M urea to 4.63M urea yields a precipitate of αs-casein and β- casein is obtained by further dilution of the supernatant to 1.7 M urea at pH of 4.7. Enzymatic hydrolysis of casein fractions was carried out at an enzyme- substrate (E: S) ratio of 1:25 The SDS-PAGE was most widely used method for analyzing protein mixtures qualitatively and RP-HPLC was useful for monitoring protein purification of Bioactive peptides (BAPs). Hydrolysed bioactive peptides of caprine milk proteins are exhibit various physiological effects in the body, such as gastrointestinal, cardiovascular, endocrine, immune, and nervous systems.
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