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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
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Original Research Articles                      Volume : 5, Issue:11, November, 2016

PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(11): 173-182
DOI: http://dx.doi.org/10.20546/ijcmas.2016.511.020


Prevalence and Genomic Characterization of Vibrio parahaemolyticus isolated from Molluscan Shellfish and their Inhabiting Water of Coastal Karnataka, India
Vilasini Udyavara*, Sangeetha Madambithara Sivadasan, Dechamma Mundanda Muthappa, Santhosh Kogaluru Shivakumaraswamy and Moleyur Nagarajappa Venugopal
Department of Fisheries Microbiology, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Mangalore, 575002, Karnataka, India
*Corresponding author
Abstract:

Vibrio parahaemolyticus is a halophilic bacterium that commonly inhabits the marine and estuarine environments. This organism is known as one of the major leading causative agent for gastroenteritis, often related to consumption of raw or undercooked seafood. In this study, molluscan shellfish collected from various estuarine environments of Dakshina Kannada and Udupi districts of Karnataka were tested and analyzed for the presence of V. parahaemolyticus. These isolates were confirmed both by phenotypic, genotypic methods and checked for presence of virulence factors too. A total of 203 isolates of Vibrio parahaemolyticus were isolated of which, 20 isolates showed positive for tdh gene. The 20 isolates were further studied for Kanagawa phenomenon, production of thermostable direct haemolysin, antibiotic susceptibility and the presence of antibiotic resistant gene, which are related to pathogenicity in humans.


Keywords: V.parahaemolyticus, haemolysin,shellfish and virulence,molluscan shellfish.

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How to cite this article:

Vilasini Udyavara, Sangeetha Madambithara Sivadasan, Dechamma Mundanda Muthappa, Santhosh Kogaluru Shivakumaraswamy and Moleyur Nagarajappa Venugopal. 2016.  Prevalence and Genomic Characterization of Vibrio parahaemolyticus isolated from Molluscan Shellfish and their Inhabiting Water of Coastal Karnataka.Int.J.Curr.Microbiol.App.Sci. 5(11): 173-182. doi: http://dx.doi.org/10.20546/ijcmas.2016.511.020
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

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