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International Journal of Current Microbiology and Applied Sciences (IJCMAS)
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2017) [Effective from January 1, 2017]For more details click here
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National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2017)
[Effective from January 1, 2017]
For more details click here

ICV 2015: 85.95
Index Copernicus ICI Journals Master List 2015 - IJCMAS--ICV 2015: 85.95
For more details click here
journals IJCMAS
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com / submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2015: 85.95
NAAS RATING 2017: 5.38

Int.J.Curr.Microbiol.App.Sci.2016.5(11): 74-85
DOI: http://dx.doi.org/10.20546/ijcmas.2016.511.008


Detecting Cronobacter Contamination in Protein Mixture and Biscuit Sample by Conventional PCR Method-A Preliminary Study
Mehal Passi, Nakul Aggarwal and Anu Priya Minhas*
Department of Biotechnology, UIET, Panjab University, Chandigarh, India
*Corresponding author
Abstract:

Cronobacter is a gram negative, non-sporulating rod shaped bacteria. It is a known contaminant of different dried milk products, mainly powdered infant formula. Cronobacter  is responsible for meningitis and enteritis especially in neonates. However elderly people and children with compromised immunity are also at risk. To detect Cronobacter contamination in food samples, various microbiological, immunological and molecular methods with variable specificity and sensitivity has been developed. This study focus on molecular detection of Cronobacter in some food products employing rpoB and ITS based PCR strategy. Total of six samples were screened with microbiological methods and positive samples were further confirmed using rpoB and ITS based conventional PCR. Although 50% tested sample were found contaminated with such bacteria, still it is a very preliminary study in such food samples requiring further investigation. ITS and rpoB sequence chosen from previous studies were conserved sequences among Cronobacter strains. Therefore probability of primer (based on these sequence) to bind at specific locus in different strains will be more. However only rpoB based primer pair successfully amplified rpoB specific sequence in three out of six food samples tested. No such amplification was observed when ITS based primer pair was used in PCR. In spite of all, such food contaminations are a matter of concern, reflecting poor handling, processing, preparation and storage procedures therefore challenging health of children and immune-compromised patients.


Keywords: Cronobacter,PCR, Enterobacteriaceae,rpoB ,ITS.
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How to cite this article:

Mehal Passi, Nakul Aggarwal and Anu Priya Minhas. 2016.  Detecting Cronobacter Contamination in Protein Mixture and Biscuit Sample by Conventional PCR Method-A Preliminary Study.Int.J.Curr.Microbiol.App.Sci. 5(11): 74-85. doi: http://dx.doi.org/10.20546/ijcmas.2016.511.008