Follow
International Journal of Current Microbiology and Applied Sciences (IJCMAS)
Home Editorial Board Current Issue Special Issue Archives Indexing Submit Article
Instruction to Authors Manuscript Charges Copyright Form Model Manuscript Process Flow (Time Frame) FAQ
Reviewer Guidelines Selection of Reviewers Join our Team of Reviewers Apply as Reviewer Reviewer Login
Editorial Review Policy Embargo Policy Ethical Expectations Consent For Identifying Information Informed Consent Manuscript Withdrawal Policy Peer Review Process Plagiarism Policy Ethical Violations Appeal Policy Against Editorial Decision Advertising Policies Authorship Policy
Access Policies Authorship Publication Policy Publication Ethics Complaints Policy Confidential Information Conflicts of Interest Policy Consent for Copyrighted Material Copyright Policy Corrections and Retractions Digital Preservation Policy Research On Human And Animal Studies Open Access Policy
Publication Ethics Contact
IJCMAS is now DOI (CrossRef) registered Research Journal. The DOIs are assigned to all published IJCMAS Articles.
Index Copernicus ICI Journals Master List 2022 - IJCMAS--ICV 2022: 95.28 For more details click here
National Academy of Agricultural Sciences (NAAS) : NAAS Score: *5.38 (2020) [Effective from January 1, 2020] For more details click here

Join as a Reviewer

Login as a Reviewer

Indexed in



National Academy of Agricultural Sciences (NAAS)
NAAS Score: *5.38 (2020)
[Effective from January 1, 2020]
For more details click here
ICV 2022: 95.28
Index Copernicus ICI Journals Master List 2022 - IJCMAS--ICV 2022: 95.28
For more details click here
Announcement

See Guidelines to Authors
Current Issues
Download Publication Certificate

Original Research Articles                      Volume : 5, Issue:10, October, 2016
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706
Issues : 12 per year
Publisher : Excellent Publishers
Email : editorijcmas@gmail.com /
submit@ijcmas.com
Editor-in-chief: Dr.M.Prakash
Index Copernicus ICV 2018: 95.39
NAAS RATING 2020: 5.38
Metrics and citations

Int.J.Curr.Microbiol.App.Sci.2016.5(10): 1114-1124
DOI: http://dx.doi.org/10.20546/ijcmas.2016.510.117


Rapid Confirmation and Molecular Identification of Alkaline Protease Producing Aspergillus awamori through Submerged Fermentation
Radhika Pilli1 and K.G. Siddalingeshwara2*
1Research and Development Centre, Bharathiar University, Coimbatore, India
2Scientific and Industrial Research Centre, Bangalore, India
*Corresponding author
Abstract:

Recent years have witnessed a phenomenal increase in the use of enzymes as industrial catalysts. Proteases constitute a very large and complex group of enzymes, widely utilized in a host of industries. Industrially important efficient alkaline protease producer was isolated from soil samples collected from different regions of Bangalore. Among the 25 isolates Aspergillus awamori KGSR 12 evolved as an potential protease producer by plate assay method and it showed 1.2 cm diameter, 46.15% hydrolysis. The protease were confirmed by TLC method by Rf value i.e 0.84.  The molecular tool used for identification of Aspergillus awamori KGSR 12 and also optimum percentage of hydrolysis (0.5%) were determined.


Keywords: Alkaline protease, Molecular confirmation, Casein and Submerged fermentation

Download this article as Download

How to cite this article:

Radhika Pilli and K.G. Siddalingeshwara. 2016. Rapid Confirmation and Molecular Identification of Alkaline Protease Producing Aspergillus awamori through Submerged Fermentation.Int.J.Curr.Microbiol.App.Sci. 5(10): 1114-1124. doi: http://dx.doi.org/10.20546/ijcmas.2016.510.117
Copyright: This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike license.

Citations
Home | Terms of Use | Copyright Form 2012-2020 Copy rights reserved IJCMAS. Powered by ThinkNEXT Technologies