National Academy of Agricultural Sciences (NAAS)
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PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
Now a day, culture maintenance is a big problem for quality control of microorganisms. The microbiologist needs to have some convenient methods for maintaining microorganisms. There are multiple methods for microbial preservation. Samples were collected from the patients in the department of microbiology at BSMC & H, Bankura, a tertiary care hospital, over a period of four months from March to June 2016. In this study performed four simple methods for preservation of bacteria such as 20%glycerol supplemented broth preserved at -20oC temperature, 0.4% semisolid agar media at 4oC, Nutrient agar at 4oC, Nutrient agar at 8oC. During preservation we performed biochemical test and observed antibiotic sensitivity pattern after each 2weeks interval. In20%glycerol broth at -20oC of Pseudomonas sp and Klebsiella sp were not viable after 7-8 weeks of preservation. In semisolid agar media all of 6 bacteria were aliveupto12 weeks. In nutrient agar medium at 4oC and at 8oC bacteria were alive up to 6-9 weeks but antibiotic sensitivity pattern showed marked change in change zone diameter for some bacteria. This large change of zone diameter was not due to human error so we cannot preserve for more than 6 weeks in Nutrient agar. From this above experiment we can conclude that preservation in 0.4% semisolid agar media at 4oC is the ideal media for the preservation of Gram positive and Gram negative bacteria.
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