National Academy of Agricultural Sciences (NAAS)
|
PRINT ISSN : 2319-7692
Online ISSN : 2319-7706 Issues : 12 per year Publisher : Excellent Publishers Email : editorijcmas@gmail.com / submit@ijcmas.com Editor-in-chief: Dr.M.Prakash Index Copernicus ICV 2018: 95.39 NAAS RATING 2020: 5.38 |
The hepatitis B virus infection is a worldwide serious clinical hitch because of its potential adverse sequelae. Detection of HBsAg can be done by different methods like Immunochromatographic (ICT) and fully automated chemiluminiscence immunoassays (CLIA). This study was undertaken to detect the presence of hepatitis B virus surface antigen (HBsAg) by Immunochromatographic test method (ICT) and eCLIA, compare them and to check the accuracy of eCLIA. 9842 serum specimens were subjected to HBsAg detection by Immunochromatographic assay (ICT) and enhanced chemiluminiscence immunoassay (eCLIA). The specimens that showed COI values between 0.9 and 35.0 by eCLIA testing were subjected to confirmatory testing using Vitros Immunodiagnostics HBsAg confirmatory kit. Out of 9842 serum specimens tested40were found reactive. Among these 28 were found reactive by both ICT and eCLIA and 12 by only eCLIA. The confirmatory test that was carried out on 12 samples showed only 2 specimens with COI> 25.4 were true positive. ROC curve analysis of eCLIA showed an area of 0.8338. The eCLIA is an accurate and good test method for the detection of HBsAg. However, weakly reactive results need to be confirmed by confirmatory test methods to rule out false positives.
Citations |
 |
 |
 |
 |
