ABSTRACT

The present study was confined to the Parangipettai estuary ecosystem in Cuddalore District, Tamil Nadu comprising of Salt Marsh (S1) and Vellar (S2). Water, sediment, seafoams and natural substrates were collected to isolate the Microorganisms.  All the collected samples were plated incubated and the Microbial colonies were identified. The water and sediment sample were collected separately and analysed for temperature, pH, dissolved oxygen, biological oxygen demand, chemical oxygen demand, salinity, total dissolved solids. A total of 41 species of microorganisms were isolated and enumerated by plating technique. Among these, 30 species were represented in S1 and 22 in S2. In this study, 27 species of microbes were recovered from sediment samples whereas water samples yielded 25 species and natural substrates with 12 species. From the sea foams, a total of 12 species were recorded. The biosynthesis of aqueous Ag+ ions by the fungus extract has been demonstrated. The findings of the present study has been reported as green chemistry approach using a fungus Fusarium oxysporum in the synthesis of silver nanoparticles at room temperature without using any harmful reducing agents such as sodium borohydrite or hydroxylamine hydrochloride and any capping or dispersing agent. Thus green synthesis of silver particles has been achieved in the present study using Fusarium oxysporum.

Keywords : Marine and Mangrove ecosystem; Isolation of marine microbes; Microbial diversity; Physico chemical parameters; Production of silver nanoparticles..