International Journal of Current Microbiology and Applied Sciences

ISSN: 2319-7706 Volume 2 Number 4 (2013) pp. 155-165

Comparison of bacterial population in industrial and agricultural soils

F. Z. Tanu* and S. Hoque

Department of Soil, Water and Environment, University of Dhaka, Bangladesh, *Corresponding author: tanu_du@yahoo.com

A B S T R A C T

This study focused on the isolation, characterization and identification of some common soil bacterial strains with the aim of investigation of population diversity and comparison of species variation in industrial and agricultural soils. A total of 15 bacteria were isolated from four soil samples collected from Dhaka Export Processing Zone (DEPZ) at Savar (SE), Tannery area at Hajaribagh (ST), Dhaka, and two agricultural fields of Dhamrai (SD) and Kushtia (SK) in Bangladesh. After characterization of the isolates morphologically, physiologically and biochemically, they were identified provisionally as different species of Bacillus, Micrococcus, and Pseudomonas. For further confirmation by using PCR technique and 16S rRNA gene sequencing 4 isolates were identified as Micrococcus luteus strain P4_3 (from SE) and Bacillus megaterium strain H2 (from ST) from industrial soils and Bacillus amyloliquefaciens strain SCSAAB0007 (from SD) and Bacillus subtilis strain 1320 (from SK) from agricultural soils. The result of this experiment showed no sharp difference in population diversity of the soils collected from industrial and agricultural soils. Under the above technique the partial 16S rRNA gene was amplified by polymerase chain reaction (PCR) using gene specific primers (universal primer) and the PCR products were purified and sequenced. Sequence analysis was done using computer based searching procedure through BLAST program.

Keywords

Soil bacteria; industrial soils; agricultural soils; PCR; 16S rRNA gene sequencing