International Journal of Current Microbiology and Applied Sciences

ISSN: 2319-7706 Volume 2 Number 3 (2013) pp. 49-56

Effect of Fish waste based Bacillus Protease in Silver recovery from waste X-Ray Films

E. Kumaran1*, A. Mahalakshmipriya2 and Sentila Rajan1

1Department of Microbiology, Dr.N.G.P. College of Arts and Science, Coimbatore- 641 038, Tamilnadu, India. 2Department of Botany, Govt. Arts College for Women, Salem, Tamilnadu, India. *Corresponding author: kumars16@yahoo.co.in

A B S T R A C T

Fish intestine harbors a lot of microflora, which comes under all groups of microorganisms. Some of them include the amylolytic, lipolytic and proteolytic forms. As the fish body is mainly proteins, the proteolytic bacteria have a major role in degrading the fish waste. In the present study, proteolytic organisms associated with fish waste were evaluated; characterized, identified and potential protease producer was screened by spot test. The selected isolates and the reference culture Bacillus subtilis 14410 were subjected for protease production in production media, Raw fish Juice, Production media plus raw fish juice and compared for highest protease producing substrates in which raw fish juice gave considerable result. Based on the activity of the protease produced by the isolated Bacillus sp., it was concluded that the strain had potential of producing an alkaline protease under pH 9 and 350C and has greater proteolytic activity profile at pH 7 to 8. The protease was partially purified and the molecular weight was determined as 45 Kda and 38Kda. It was used as a major constituent in waste X-ray Silver recovery. Compared to Bacillus subtilis 14410, isolated Bacillus sp. from fishwaste yield maximum protease and show higher activity in Waste X-ray silver recovery.

Keywords

Fish intestine; proteolytic organisms; spot test; Bacillus subtilis 14410; Waste X-ray; silver recovery.